Evaluation of a graded exercise test to determine peak fat oxidation in individuals with low cardiorespiratory fitness

The aims of this study were to develop a graded exercise protocol employing four-minute stages to observe whether steady-state gaseous exchange was attained within four-minutes and to assess the graded test’s surrogate validity and day-to-day reliability to determine rates of peak fat oxidation during exercise (PFO) in individuals with low cardiorespiratory fitness. This study was a cross-over design where sixteen healthy adults first completed an incremental graded exercise test (GE) followed by three short continuous exercise (SCE) sessions all in an overnight fasted-state (11 ± 1 h). The GE test was completed to determine rate of peak fat oxidation during exercise (PFO) and FATMAX i.e. the exercise intensity PFO occurred at (measured in absolute watts). The three SCE sessions were completed in a randomised order on separate days and involved completion of the graded test to the stage: 1) preceding (SCEpre); 2) equal to (SCEequal); or 3) after (SCEpost), PFO was attained, where participants then continued to cycle for 10-minutes at that respective intensity. Expired gases were sampled at minutes 3-4, 5-6, 7-8 and 9-10 and substrate oxidation rates were assessed via indirect calorimetry

Dataset for "Biological sex and aerobic capacity are key determinants of peak fat oxidation rates during exercise"

The dataset includes demographic data of the participants (n=115) and data from the three trials they participated in. The types of data that were collected during the trials are as follows: Trial A and Trial B followed identical protocols that involved anthropometric measurements, resting metabolic rate, a resting venous blood sample (to look at various plasma metabolite and hormone concentrations) and the completion of a graded exercise test to determine PFO and FATMAX by indirect calorimetry; at Trial C, body composition was assessed (via a dual energy x-ray absorptiometry scan) and optional adipose tissue and/or skeletal muscle biopsies were obtained (Biopsy data not included). All trials were completed in an overnight fasted-state

Datset for "Resting skeletal muscle ATGL and CPT1B are associated with peak fat oxidation rates in men and women but do not explain observed sex differences"

The dataset includes demographic data of the participants who opted for adipose tissue and/or skeletal muscle biopsies (n=36) recruited as part of a larger study (n=115). Additionally, data from the three trials they participated in are included. The types of data that were collected during the trials are as follows - Trial A and Trial B followed identical protocols that involved anthropometric measurements, resting metabolic rate, a resting venous blood sample (to look at various plasma metabolite and hormone concentrations) and the completion of a graded exercise test to determine PFO and FATMAX by indirect calorimetry. At Trial C, body composition was assessed (via a dual energy x-ray absorptiometry scan) and optional adipose tissue and/or skeletal muscle biopsies were obtained. The adipose tissue and muscle biopsy data revolves around the content of several key proteins involved in fat metabolism in adipose tissue and skeletal muscle that may regulated fat use during exercise. All trials were completed in an overnight fasted-state

Dataset for the day-to-day reliability of peak fat oxidation and FATMAX

The dataset includes demographic data of the participants (n=115) and data from the three trials they participated in. Types of data that were collected during the trials are as follows - Trial A and Trial B followed identical protocols that involved anthropometric measurements, resting metabolic rate, a resting venous blood sample and the completion of a graded exercise test to determine PFO and FATMAX by indirect calorimetry. At Trial C, body composition was assessed (via a dual energy x-ray absorptiometry scan) and optional adipose tissue and/or skeletal muscle biopsies were obtained. All trials were completed in an overnight fasted-state.Please see attached readme file

Dataset for 'Prior exercise alters the difference between arterialised and venous glycaemia – implications for blood sampling procedures.' [Data Collection]

Oral glucose tolerance and insulin sensitivity are common measures, but are determined using various blood sampling methods, employed under many different experimental conditions. This study established whether measures of oral glucose tolerance and oral glucose-derived insulin sensitivity (ISI) differ when calculated from venous versus arterialised blood. Critically, we also established whether any differences between sampling methods are consistent across distinct metabolic conditions (after rest versus after exercise). Ten healthy men completed two trials in a randomised order, each consisting of a 120-minute oral glucose tolerance test (OGTT), either at rest or post-exercise. Blood was sampled simultaneously from a heated hand (arterialised) and an antecubital vein of the contralateral arm (venous). Under both conditions, glucose time-averaged area under the curve was greater from arterialised compared to venous plasma but importantly, this difference was larger after rest relative to after exercise (0.99 ± 0.46 versus 0.56 ± 0.24 mmol/L respectively; p < 0.01). OGTT-derived ISIMatsuda and ISICederholm were lower when calculated from arterialised relative to venous plasma and the arterialised-venous difference was greater after rest versus after exercise (ISIMatsuda: 1.97 ± 0.81 versus 1.35 ± 0.57 au, respectively; ISICederholm : 14.76 ± 7.83 versus 8.70 ± 3.95 au, respectively; both p < 0.01). Venous blood provides lower postprandial glucose concentrations and higher estimates of insulin sensitivity, compared to arterialised blood. Most importantly, these differences between blood sampling methods are not consistent after rest versus post-exercise, preventing standardised venous-to-arterialised corrections from being readily applied. Registered under ClinicalTrials.gov Identifier no. NCT02852044. Supplementary (individual participant) data are included in this dataset for reported outcome measures.As described in the manuscript, ten healthy men completed a preliminary assessment of body composition and an incremental cycling exercise test, before two main trials, where a 120-minute oral glucose tolerance test (OGTT) was completed either after rest or after moderate intensity cycling. Arterialised and venous blood samples were collected simultaneously at baseline (after a minimum 10 h fast) and at 15 minute intervals post glucose ingestion. To obtain arterialised samples, participants placed their dominant hand into a heated-air box set to a constant temperature of 55°C, and after 20 minutes, an intravenous cannula was fitted in the heated dorsal hand vein (retrograde). To obtain venous samples, an intravenous cannula was fitted in the antecubital fossa of the contralateral arm (antegrade). All samples for a given participant were analysed in batch within the same assay/plate

Evaluation of a graded exercise test to determine peak fat oxidation in individuals with low cardiorespiratory fitness

The aims of this study were to develop a graded exercise protocol employing four-minute stages to observe whether steady-state gaseous exchange was attained within four-minutes and to assess the graded test’s surrogate validity and day-to-day reliability to determine rates of peak fat oxidation during exercise (PFO) in individuals with low cardiorespiratory fitness. This study was a cross-over design where sixteen healthy adults first completed an incremental graded exercise test (GE) followed by three short continuous exercise (SCE) sessions all in an overnight fasted-state (11 ± 1 h). The GE test was completed to determine rate of peak fat oxidation during exercise (PFO) and FATMAX i.e. the exercise intensity PFO occurred at (measured in absolute watts). The three SCE sessions were completed in a randomised order on separate days and involved completion of the graded test to the stage: 1) preceding (SCEpre); 2) equal to (SCEequal); or 3) after (SCEpost), PFO was attained, where participants then continued to cycle for 10-minutes at that respective intensity. Expired gases were sampled at minutes 3-4, 5-6, 7-8 and 9-10 and substrate oxidation rates were assessed via indirect calorimetry